长期应用复合维生素B对大鼠福尔马林痛的影响

Effect of vitamin B complex on nociception of formaldehyde model in rats

目的观察一段时间连续全身应用复合维生素B(VitBCo)对大鼠福尔马林痛的影响。方法雄性SD大鼠随机分为4组,每组6只。VitBCo由VitB1,VitB6,VitB12按1:1:0.01比例配制,分别以50-200mg·kg-1腹腔给药。Ⅰ组为生理盐水+福尔马林组,Ⅱ组为VitBCo(50mg)+福尔马林组,Ⅲ组为VitBCo(100mg)+福尔马林组,Ⅳ组为VitBCo(200mg)+福尔马林组。Ⅰ组大鼠连续7d腹腔注射生理盐水,Ⅱ,Ⅲ,Ⅳ组连续7d腹腔注射VitBCo所有大鼠第7天腹腔注射药物1h后开始在左后爪底皮下注射0.37%甲醛100μL,记录各组大鼠第Ⅰ期(5-10min)、第Ⅱ期(15-70min)舔咬注射爪的时间;4h后分在膝关节水平截取双后爪,比较注射爪和对照爪重量的差异。结果与Ⅰ组比较,Ⅱ,Ⅲ,Ⅳ组大鼠第Ⅰ期舔、咬注射爪的时间没有显著性差异,第Ⅱ期的舔、咬时间依次减短,但仅Ⅳ组有显著性差异;注射爪和对侧爪重量的差异依次减小,Ⅲ,Ⅳ组有显著性差异。结论VitBCo可改善大鼠的福尔马林Ⅱ期痛和炎症水肿。

OBJECTIVE To observe the effect of vitamin B complex on the nociception of formaldehyde model. METHODS Twentyfour adult male SD mrs were divided randomly into four equal groups. The mrs in group Ⅰ were treated with saline aht the mrs in Ⅱ , Ⅲ group and IV were treated with vitamin B complex 50,100 and 200 mg·kg^-1 ,respectively for 7 days.Formaldehyde （ 0.37%, 100 μL） was injected subcutaneously into the left hindpaw at 1 h after the last dose in the seventh day. The time the rats licked the injected hindpaw was recorded from 0 to 15 min （the first phase） ,and from 15 to 70 min（the second phase）. And 4 h later,hindpaw oedema was evaluated with the weight difference between the injected and the contralateral non-injected hindpaw. RESULTS The chroic i. p of vitamin B complex in groups Ⅱ ,Ⅲ and Ⅳ didn＇t change the licking-paw time in the first phase of formalin test, but significantly reduced the licking-paw time in the secend phase and the weight difference. CONCLUSION Vitamin B complex inhibites the hyperalgesia and inflammatory pain in the second phase of mrs formaldehyde model and reduced the pawedema.