乙醇肝损伤小鼠肝脏的基因表达谱研究

Gene expression profile of alcoholic liver-injured mice using cDNA microarray

目的研究乙醇肝损伤小鼠肝组织的基因表达谱,筛选乙醇肝损伤相关基因,并探讨其肝损伤机制。方法①将实验小鼠分为正常对照组与乙醇肝损伤组,分别提取2组小鼠肝脏mRNA,经反转录后分别用Cy3,Cy5荧光标记,获得2组动物来源的cDNA探针;②cDNA探针与联合基因公司BiostarM-141S小鼠基因表达谱芯片杂交,结果由扫描仪扫描并用统计软件进行分析。结果与正常对照组相比,乙醇肝损伤组有117条基因发生差异性表达(0.83%),其中46条基因表达上调,另外71条基因表达下调。结论利用表达谱芯片结合实验动物模型能大规模、高通量地研究乙醇肝损伤相关基因,对进一步阐明乙醇对肝脏的损伤机制具有重要的意义。

OBJECTIVE: To study the gene expression profile of alcohol liver injuried mice and screen the differentially expressed genes that relate to liver injury formation by alcohol on a large scale using cDNA microarrays. METHODS: The experimental mice were divided into two groups: one was control group and the other was alcohol-administrated group. The mRNA in the livers of the two groups was extracted respectively and reversely transcribed to cDNA with the incorporation of different fluorescent-labeled dUTP (Cy3-dUTP, Cy5-dUTP) as the hybridization probes. The mixed probes were hybridized to the cDNA microarrays. The fluorescent signal values were acquired by scanner and analyzed by statistical software. RESULTS: Among the 14 100 target genes, 117 genes were differentially expressed of in which 46 genes were up-regulated and another 71 genes were down-regulated. They were closely related to a range of biological functions. CONCLUSION: Using cDNA microarray and experimental animal modeling technique can study the differentially expressed genes of alcoholic liver injury in mouse on a large scale. It is useful for further investigation of the injury molecular mechanism of alcohol.