实体瘤细胞LI、ER及体外化疗敏感性测定的临床意义

CLINICAL MEANING OF THYMIDINE LABELING INDEX AND ESTROGEN RECEPTOR IN VITRO DRUG SENSITIVITY OF SOLID CANCER CELLS

为了进一步认识肿瘤的生物特性,寻找快速简便的化疗预测方法及估计预后,本研究测定了40例人恶性肿瘤氚化胸腺嘧啶核苷标记指数(LI)及雌激素受体(ER),同时以LI结合Giemsa染色,观察其中34例肿瘤原代培养细胞对5-Fu的敏感性。

The thymidine labeling index(LI) and estrogen receptor (ER) of 40 cancer caseswas determined. The fesult of LI was 0-14 and the geometric mean 3. 2 (gastric cancer2. 4, colon cancer 3. 6 and breast cancer 3. 4). The positive rate of cancer ER was 39%(gastric cancer 44%, colon cancer 33% and breast cancer 45%). Primary culture of 34cases of cancer was performed in vitro with 73. 5% successful rate, the explants startedto grow averagely on 10.3 day and showed various sensitivity to 5-FU. The cancerLI, ER and cell culture can reflect tbe biological characteristics of cancer from differentangles,and may distinguish distinct cancer subgroups or may classify cancer accordingto biological behavior. The in vitro drug sensitive test and its relation with D typeER cell ratio may provide basis for selecting chemotherapy, further study is needed forits theoretical meaning and its application.