淫羊藿提取物抗抑郁作用研究

Antidepressant effect of Epimedium brevicornum extracts

目的研究淫羊藿提取物的抗抑郁作用。方法采用行为绝望模型悬尾试验(TST)和强迫游泳试验(FST)研究淫羊藿提取物对小鼠行为、脑内单胺氧化酶A(M AO-A)、单胺氧化酶B(M AO-B)活性与肝脏中M AO-A和M AO-B活性及丙二醛(M DA)水平的影响;采用利血平拮抗模型探讨淫羊藿提取物可能存在的抗抑郁作用途径。结果淫羊藿提取物(25、50、100、200 m g/kg)能显著缩短TST和FST小鼠悬尾和游泳不动时间,显著抑制TST小鼠脑和肝组织M AO-A和M AO-B活性,逆转肝组织M DA水平的升高。淫羊藿提取物对利血平所致小鼠体温的下降无明显改善作用。结论淫羊藿提取物具有一定抗抑郁作用。

Objective To study antidepressant effects of Epimedium brevicornum extracts. Methods Behavioral despair models of male mice, tails suspension test （TST）, and forced swimming test （FST） were used to evaluate the effects of E. brevicornum extracts on behavioral, monoamine oxidas （MAO-A） and monoamine oxidase B （MAO-B） activities in brain and liver tissue, and MDA level in liver tissue of mouse. Reserpine antagonistic model was also used to investigate possible antidepressant mechanisms of E. brevicornum extracts. Results The extracts of E. brevicornum （25, 50, 100, and 200 mg/kg） significantly reduced the duration of murine immobility in TST and FST, inhibited MAO-A and MAO-B activities in brain and liver of mice, and reversed the elevated liver MDA level in mice in TST. There was no significant amelioration in the decreases of body temperature in mice of Reserpine antagonistic model. Conclusion E. brevicornum extracts possesses the definite antidepressant properties.