甘草黄酮对S_(180)和H_(22)荷瘤小鼠肿瘤细胞DNA和RNA的影响

Effect of Glycyrrhiza flavonoids on DNA and RNA in tumor cell of S_(180) and H_(22) tumor-bearing mice

目的观察甘草黄酮对S180和H22荷瘤小鼠肿瘤细胞DNA及RNA的影响。方法S180和H22荷瘤小鼠随机分为甘草黄酮高、中、低剂量(25、11.25、5.58 m g/kg)组,阳性对照(环磷酰胺25 m g/kg)组和阴性对照(生理盐水)组,各组均sc给药。运用激光扫描共聚焦显微镜技术与吖啶橙(AO)荧光探针技术结合检测单一肿瘤细胞DNA和RNA的变化情况。结果不同剂量甘草黄酮组和环磷酰胺组的DNA及RNA荧光强度较生理盐水组有不同程度的减弱;甘草黄酮高、低剂量组RNA/DAN值与生理盐水组比较差异无显著性;甘草黄酮中剂量组、环磷酰胺组RNA/DNA值较生理盐水组有非常显著提高(P<0.01)。结论甘草黄酮与肿瘤细胞DNA和RNA结合后抑制其进一步复制与合成,可能是其抗肿瘤作用的途径之一。

Objective To observe effects of Glycyrrhiza flavonoids on DNA and RNA in tumor cell of S180 and H22 tumor-bearing mice. Methods S180 and H22 mice were randomly divided into Glycyrrhiza flavonoids （25, 11.25, and 5.58 mg/kg） groups, positive control （cytoxan 25 mg/kg） group, and negative control （NS） group, whom were given drugs by sc. DNA and RNA in tumor cells were examined, respectively by Laser Scanning Confocal Microscope and fluorescent probe of acridine orange （AO） technology. Results All different dosage of Glycyrrhiza flavonoids, cytoxan reduced the brightness of fluorescence of DNA and RNA ; low and high dosage of Glycyrrhiza flavonoids had no significant effect on the fluorescence pixels of RNA/DNA; Both middle dosage of Glycyrrhiza flavonoids and cytoxan had significant effect on the fluorescence pixels of RNA/DNA （P〈0. 01）. Conclusion The duplication and synthesis of DNA and RNA in tumor cells are restrained after Glycyrrhiza flavonoids binding with them. This effect may be one of the mechanisms of antitumor effect of Glycyrrhiza flavonoids.