毛头鬼伞富铬深层发酵条件的优化

Optimum of Submerged Fermentation Parameters for Chromium-rich Mycelia of Coprinus comatus*

在筛选出最佳发酵培养基的基础上,对毛头鬼伞富铬深层发酵培养条件进行了优化.以有机铬产量为主要指标,筛选出的最佳发酵培养条件为:CrCl3·6H2O的添加量为200mg/L,培养基初始为自然pH,接种量为8%～10%,装液量为80mL/250mL三角瓶,转速100r/min,26℃恒温培养5d,有机铬的产量达1597.88μg/100mL发酵液,铬富集率达9.09%.

Submerged fermentation parameters for the chromium-rich mycelia of Coprinus comatus was studied in optimal medium. According to the yield of organic Cr, the optimal fermentation parameters were obtained as follows： the amount of CrCl3 6H2O in medium was 200mg/L; the inoculation volume was 8%～10%; the aeration ratio was 80：250 （ medium volume： flask volume） ; the cultural period was 5 days at 26℃ and at 100r/min. The highest yield of organic Cr in mycelia of Coprinus comatus was 1597.88μg/100mL and the enrichment rate of Cr was about 9.09%.