利用拮抗试验和RAPD对灵芝属菌株进行分类研究

Study on Classification of Strains of Ganoderma by Anatagonistic Effect and Rapd

利用RAPD和拮抗试验对来自国内外的10个灵芝属菌株进行了遗传多样性分析。RAPD分析的结果表明,在0·560的相似性水平上分成3个组,第1组包括密纹薄芝、灵芝、灵芝(信州菌株)和两个无柄灵芝菌株,第2组包括灵芝(G·sp·)、近拟鹿角灵芝和紫芝,第3组是树舌灵芝。这一结论与传统分类学的结论基本一致。当相似性水平达到0·800的时,上述10个菌株聚成8组,与传统的分类学中种的划分几乎一致。比较两种方法所得的结果发现,它们的结论是一致的。因此认为拮抗试验在灵芝亲缘关系的初步鉴定中是有效的,RAPD在灵芝种间鉴定时是有效的,RAPD具有用于种内鉴定的可能性。

Genetic diversity among 10 strains of Ganoderma from different provinces of China and other countries were analyzed by RAPD and antagonistic effects, respectively. The dendrogram derived from RAPD data indicated that those strains could be divided into three distinct groups at the similarity level of 0. 560： the first group included Ganoderma tenue, G. resinaceum, G. lucidum （Xinzhou strain） and G. lucidum; the second one included G. sp. , G, subamboinense and G. sinense ; the last one did G. applanatum. The result was in near accordance with that of traditional classification of Ganoderma. And the above 10 strains could be divided into 8 groups at the similarity level of 0. 800, and the result was nearly in accordance with that of traditional classification. Comparing with the results by the two methods, we found that they were in accordance with each other. The present study indicated that （ 1 ） the method of antagonistic effect was effective in the preliminary identification of strains of Ganoderma; （2） RAPD was effective for the classification of the species in C, anoderma, and RAPD might be used to distinguish different strains of the same species.