变色栓菌产锰过氧化物酶的条件优化

The Optimization of Manganese Peroxidase Production by Trametes versicolor

研究了多种培养基组分及培养条件对变色栓菌产锰过氧化物酶(MnP)的影响。当培养基中果糖浓度为20g/L,酒石酸铵浓度为10mmol/L,吐温80浓度为1·0g/L,MgSO4·7H2O为0·43g/L,最终pH为4·5,500mL三角瓶装液量为100mL,接种量为10片(8mm)菌苔,培养温度为30℃,转速为280r/min时,MnP的活力有了很大程度的提高,最高酶活力可达2,270U/L。

The effect of different conditions on manganese peroxidase production by Trametes versicolor was studied. When cultivation was at 30℃, 280r/min for 7 days, with inoculation of 10 pieces of Trametes versicolor （ФSmm） in 100mL culture medium of 500mL shake flask, containing 10g/L fructose, 10mmol/L ammonium tartrate, 1. 0 g/L tween 80, 0. 43 g/L MgSO4·7H2O, pH 4. 5, manganese peroxidase activity reached 2. 270U/L.