Vc、β-葡聚糖和藻粉对中国对虾幼虾生长、成活率及免疫酶活性的影响

The effect of Vc,beta-glucan and algae powder on growth, survival rate and immune enzyme activities of Penaeus chinensis juvenile

在中国对虾幼虾的基础饲料中分别添加不同剂量的维生素C(0、0.015%、0.030%、0.045%、0.060%)、β-葡聚糖(0、0.050%、0.075%、0.100%)和藻粉(0、2%、5%、7%),未添加组为对照组,探讨维生素C(Vc)、β-葡聚糖和藻粉对中国对虾幼虾成活率、相对增长(重)率及肌肉酸性磷酸酶(ACP)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、溶菌酶(LSZ)活性的影响。室内网箱饲养40 d试验结果表明,饲料中添加Vc后,0.030%组幼虾相对增重率和CAT活性显著高于对照组(P<0.05),0.045%组ACP、CAT活性显著高于对照组(P<0.05),但Vc对SOD活性没有显著性影响,0.015%组LSZ活性极显著高于对照组(P<0.01);0.075%β-葡聚糖组的相对增重率、SOD活性显著高于对照组(P<0.05);添加7%藻粉能极显著提高相对增长(重)率(P<0.01),但ACP和LSZ活性呈极显著下降(P<0.01),添加5%藻粉能显著提高CAT活性(P<0.05)。

Penaeus chinensis juvenile were fed on basal diets supplemented with different levels Vc （0,0. 015%, 0.030% ,0.045% ,0.060%）, beta-glucan （0,0.050% ,0.075% ,0. 100% ）and algae powder（0,2% ,5% ,7% ）for 40 days respectively. The shrimp fed on basal diet were regarded as control . Each group was treated with triplicate. Growth, survival rate and activities of acid phosphates（ACP）, catalase （CAT）, superoxidase（SOD） and lysozyme（LSZ） were measured. The relative body length gain and CAT activity of 0. 030% Vc group were significantly higher than those of the control（ P 〈 0.05）. The activities of ACP and CAT of 0.045% Vc group were enhanced（ P 〈 0.05）. The activities of SOD were not affected by Vc supplement（ P 〈 0.05）.The LSZ activities of 0.015% Vc group were significantly increased （P 〈 0.01）. The relative body length gain and SOD activity of 0. 075 % beta-glucan group were significantly higher than those of the control（ P 〈 0.05）. The relative body length and weight gain of 7 % algae powder group were significantly increased （ P 〈 0.01 ）, but the activity of ACP and LSZ of the group was decreased （P 〈 0.05）. The activities of CAT of 5% algae powder were enhanced（ P 〈0.05）.