转基因三倍体毛白杨的抗虫机理研究

Studies on insect-resistant mechanism of transgenic triploid of Chinese white poplar

以高抗虫、中等抗虫和低抗虫转双抗基因三倍体毛白杨(7号、10号和26号)的叶片饲养4龄的杨扇舟蛾幼虫,4 h、12 h2、4 h4、8 h7、2 h和96 h后分别测定其中肠内的解毒酶和消化酶活性。结果表明,用7号叶片饲养的幼虫中肠的酯酶、羧酸酯酶和多功能氧化酶受到明显的抑制,10号叶片饲养的幼虫次之,26号叶片饲养的幼虫的这些酶抑制不太明显。中肠的淀粉酶也是如此。但谷胱甘肽S-转移酶和蛋白酶活性变化比较复杂,24h时出现峰值,随后下降。其中7号叶片饲养的幼虫最明显,10号叶片饲养的幼虫次之,但26号叶片饲养的幼虫的蛋白酶变化不明显。转基因杨叶片对幼虫中肠的解毒酶和消化酶活性的抑制可能是其杀虫机制。

The activities of detoxification enzymes and digestive enzymes of Clostera anachoreta （Fabricius） instar larvae feeding on leaves of three kinds of insect-resistant clones of transgenic Triploid of Chinese White Poplar, after 4,12,24,48,72 and 96 hours, were investigated. The results showed that, feeding On the clone 7, the activity of esterase, carboxylesterase and mix-function oxidase in the midgut of the larvae were tremendously decreased. Feeding on the clone 10, those results were less than the results above, and there were few changes on the larvaes which feeded on the clone 26. Moreover, the changes of the amylase in the midgut of larvaes were the same as above. However, the activities of glutathione s-transferase and proteinases were complex, increased remarkably after 24 hours feeding on the clone 7, then declined rapidly. Same changes were taken place on the larvaes feeding on the clone 10. There are many slight changes on glutathione s-transferase of the larvaes , feeding on the clone 26, no changes on the proteinases in the midgut. Thus the detoxification enzymes and digestive enzymes in the midgut of the larvaes were inhibited. This may be the main mechanisms of the transgenic Triploid of Chinese White Poplar.