反相高效液相色谱法测定人血清中奈韦拉平浓度

Determination of serum nevirapine by RP-HPLC

目的:建立RP-HPLC法测定人血清中奈韦拉平的浓度.方法:以盐酸左氧氟沙星为内标,色谱条件:ZORBAX Eclipse XDB-C8色谱柱(150 mm×4.6 mm,5μm),流动相为15 mmol·L-1磷酸氢二铵溶液(pH=7.5)-乙腈(80:20);流速1.5 mL·min-1;检测波长295 nm.结果:线性范围为0.05～8μg·mL-1,相关系数r=0.999 3.结论:测定方法简便、准确、专属性好.

Objective： To establish a RP-HPLC method to assay serum nevirapine. Methods： The HPLC conditions were as follows： ZORBAX Eclipse XDB-C8 column （4.6 mm × 150 mm, 5 μm）, the mobile phase consisted of 15 mmol· L^-1 （NH4）2HPO4 （pH 7.5 adjusted by phosphate acid）-acetonitrile （80：20） with the flow rate of 1.5 mL·min^-1 and the detection wavelength at 295 nm. Levofloxacin was used as an internal standard. Results： The linear curve was in the range of 0.05 - 8 μg·mL^-1 （ r=0. 999 3 ）. Conclusion： The RP-HPLC method is simple, accurate and reliable.