DON毒素作用后小麦愈伤组织盐溶性蛋白质变化

The protein profile of wheat callus after treated with deoxynivalenol

用0.1 m g.mL-1的脱氧雪腐镰刀菌烯醇(DON)处理对赤霉病抗性程度不同的5个小麦品种(苏麦3号、望水白、扬麦158、宁麦6号及安农8455)成熟种子愈伤组织,分别于6、12、24 h后用FPLC方法分析组织中的盐溶性蛋白(细胞壁蛋白)组分,探讨小麦愈伤组织中盐溶性蛋白对DON的反应。结果表明:所有供试品种的组织提取液中均含有洗脱体积为8.00(P 8)、18.20(P 18)、20.20(P 20)mL的蛋白吸收峰。DON处理后,抗病品种中这些吸收峰明显增高,而感病品种中这些吸收峰变化不明显或有下降趋势;抗病品种苏麦3号的愈伤组织细胞壁蛋白图谱变化与过氧化氢处理对照样品的趋势相同,说明小麦愈伤组织对DON反应中的蛋白变化可能与过氧化氢有关。

The callus of five wheat varieties （Sumai 3, Wangshuibai, Yangmai 158, Ningmai 6 and Annong 8455） with different resistance to wheat scab were treated with deoxynivalenol （DON, 0.1 mg · mL^-1）. After 6, 12, 24 h, salt extraction of these callus were examined by FPLC gel-filtration. Results showed that 4 protein peaks （designated P8, P18, P20, P21） were bigger than control in resistant varieties, when samples were treated with DON, but these protein peaks were decreased in susceptible varieties. When the samples of non-treated resistant varieties were incubated with H2O2, the profiles were very similar to those of DON treated ones. Results indicated that these proteins might be the product of cross-linking mediated by H2O2 and catalyzed by peroxidase during the resistant response of wheat callus against deoxynivalenol.