宫颈癌患者单纯疱疹病毒Ⅱ型抗体和人乳头状瘤病毒核酸检测的相关性

CORRELATION OF HSV-2 ANTIBODY AND HPV DNA SEQUENCES IN CERVICAL CARCINOMA

自1966年Naib首次提出单纯疱疹病毒Ⅱ型(HSV-2)与宫颈癌发生有关以来,经过20多年的研究,虽然大量的资料支持这一设想,但也存在许多不能解释的问题,因此人们对HSV-2的单一作用开始怀疑。1977年Zur Hausen提出人乳头状瘤病毒(HPV)可能是宫颈癌的又一危险因素。1982年Zur Hausen又提出HSV-2作为“启动子”与HPV协同致癌学说,但此假设尚缺乏实验依据,仅是理论上的推测。本实验通过对宫颈癌患者血清HSV-2抗体及癌活检组织HPV DNA的检测,试图探讨HSV-2和HPV的可能联系。

To investigate the possible role and correlation of herpes simplex virus type Ⅱ(HSV-2) and human papilloma virus(HPV) in the causation of cervical carcinoma,theserum HSV-2 antibody in cervical carcinoma patents and the HPV DNA sequencewere determined by indirect immunoperoxidase assay (IPA) and by DNA dot blothybridization respectively.It was showed that the percentage of HPV 16 DNA sequencesin cervical carcinoma was higher than that of RPV 11 and 18, the HSV-2 antibodytiter in cervical carcinoma group and in non-cervical careinoma group showed nosignificant difference, and the HSV-2 antibody titers in HPV (including HPV 16, 18,11) DNA positive and negative patients were approximately the same. The resultswere discussed.