薄层扫描法测定大豆磷脂及蛋磷脂中磷脂酰胆碱的含量

TLC Determination of Phosphatidylcholine in soybean Phospholipid and egg Phospholipid

目的:应用薄层色谱扫描法测定样品中磷脂酰胆碱的含量。方法:薄层板:硅胶 G;展开剂:乙酸乙酯-二氯甲烷-甲酸(3:4:1.5);显色剂:5%磷钼酸无水乙醇溶液。检测波长:535 nm,参比波长700 nm。结果:线性范围:10～35μg(r=0.9954),平均回收率95.3%。结论:该法简便、快速、经济,可作为磷脂含量控制的有效方法。

Objective：Determination of phosphatidylcholine by TLC. Methods：The silica gel plate was used, with CH3 COOC2 H5 - CH2Cl2 - HCOOH （ 3 ： 4 ： 1.5 ） as eluent; The detection wavelength was 535 nm, reference wavelength was 700 nm. Results. A good linearity was shown in the range of 10 - 35μg（ r = 0. 9954）, the average recovery was 95. 3%. Conclusion：This method is simple, rapid and economical for the quality control of phosphatidylcholine.