双波长薄层扫描法测定复方磺胺甲噁唑片中磺胺甲噁唑和甲氧苄氨嘧啶的含量

Determination of Suffamethoxazole and Trimethoprim in Compound Sulfamethoxazole Tablets by Dual Wavelength TLC Scanning

目的:测定复方磺胺甲(口恶)唑片中磺胺甲(口恶)唑(SMZ)和甲氧苄氨嘧啶(TMP)的含量。方法:选用展开剂为氯仿-甲醇(9:1),双波长薄层反射锯齿扫描,线性参数:SX=7,狭缝:0.4 mm×0.4 mm,灵敏度:X1,扫描速度为12 mm·min^(-1),测定波长:SMZ λ_s=271nm,λ_R=335nm;TMPλ_s=275nm,λ_R=325nm。结果:SMZ 与 TMP 的含量线性范围分别是2.00～10.00μg(r=0.9995)和0.40～2.00μg(r=0.9996),平均加样回收率分别为99.2%,RSD 为0.46%和99.6%,RSD 为0.80%。结论:本测定法简便、快速、准确、重现性好,可作为复方磺胺甲(口恶)唑片中 SMZ 和 TMP 的含量测定方法。

Objective：To establish a method using dual wavelength TLC -scanning for the determination of sulfamethoxazole （SMZ） and trimethoprim （TMP） in compound sulfamethoxazole tablets. Method： Developer of selection was chloroform- methanol（9：1 ） ,wavelength of the SMZ was λR =271 nm,λR =335 nm;wavelength of the TMP was λR = 275 nm,λR =325 nm. The determination were carried out with the reflection zigzag scanning,SX =7 ,slit =0. 4 mm × 0. 4 mm,sensitivity：X1 ,scan speed： 12 mm × min^-1. Result：The linear ranges of SMZ and TMP were 2. 00 - 10. 00μg （r =0. 9995） and 0. 40 -2. 00μg（r =0. 9996） respectively,the average recovery rates（n =6）of SMZ and TMP were 99. 2% （ RSD = 0. 46% ） and 99. 6% （ RSD = 0. 80% ） respectively. Conclution： The method was proved to be simple, rapid, accurate and reproducible.