银耳碱提多糖抗氧化活性的研究

Study on antioxidation activities of polysaccharides extracted from Tremella fuciformis Berk.

目的对从银耳孢子发酵物中用碱液提取得到的4个多糖组份的抗氧化活性进行研究。方法采用清除羟基自由基实验、清除超氧阴离子自由基实验、抗H2O2诱导的红细胞氧化溶血实验考察4个多糖组份的抗氧化活性。结果在H2O2引起的红细胞溶血试验中,以蒸馏水溶血率为100%,TFFB-A、TFFB-B、TFFB-C、TFFB-D溶血率分别为21.4%、31.2%、28.5%、25.6%;在对超氧阴离子自由基(O2.)的清除作用实验中,TFFB-A、TFFB-B、TFFB-C、TFFB-D最大清除率分别为21.4%、28.4%、36.6%、53.0%;在清除羟基自由基实验中,TFFB-A、TFFB-B、TFFB-C、TFFB-D的EC50分别为233.6mg/L、603mg/L、191mg/L、195mg/L。结论4个多糖组分均具有一定的清除.OH、O2.和抑制红细胞溶血的活性。

Object： To study antioxidant effect of gradient sodium hydroxide extracting polysaccharides from Tremella fuciforrnis Berk fermentation products. Methods： The antioxidant reaction of the four polysaccharides was studied by three experiments： inhibiting red cells hemolysis by H2O2, eliminating superoxide anion free radical （O2^-·） and scavenging hydroxyl free radicals（·OH）. Results： The inhibiting ratio of TFFB-A, TFFB-B, TFFB-C, TFFB-D for red cells hemolysis are 21.4, 31.2, 28.5 and 25.6% respectively; the eliminating ratio for O2^-· are 21.4 %, 28.4 %, 36.6 96, 53.096 respectively; They can also scaveng hydroxyl free radicals（·OH）, the consistence of eliminating 50% of all free radicals（EC50）are 233.6, 603, 191 and 195mg/L respectively. Conclusion： The all four polysaccharides from Tremella faciformis Berk fermentation products have a certain capability of antioxidation.