当归药物血清逆转K562/A02细胞对阿霉素耐药性的初步观察

Primary observation of reversal of multidrug resistance in K562/A02 cells with Radix Angelicae sinensis

目的以白血病多药耐药细胞系K562/A02为对象,观察当归能否逆转其对阿霉素的耐药性。方法采用MTT法观察当归药和血清对阿霉素细胞抑制率的影响(IC50)。结果经MTT法发现当归药物血清能增加K562/A02对阿霉素的敏感性,使阿霉素半数抑制浓度(IC50)由14.9mg/L,降至9.17mg/L,部分逆转了K562/02对阿霉素耐药性,逆转倍数为1.63倍。而当归对K562/S敏感细胞系无上述作用。结论当归能部分逆转K562/A02细胞对阿霉素的耐药性。

Objective： To study effective affect Radix Angelic^e sinensis as MDR reversing agent. Method： To test the Changes of adriamycin （ADM） ＇S IC50 in human leukemia multidrug resistance cell line K562/A02 by MTT assay. Result： The drug-resistance of the MDR cell line could be party reversed by Radix Angelicae sineusis. Conclusion： Radix Angelicae sinensis can partly reverse multidrug resistance in leukemia.