中药热毒清对人巨细胞病毒抑制作用的实验与临床研究(英文)

Experimental and clinical study on inhibitory effects of Reduqing on human cytom egalovirus

目的:探讨中药热毒清对人巨细胞病毒(HCMV)体内外的抑制作用及机制。方法:采用定量逆转录聚合酶链反应(RT-PCR)方法检测中药热毒清干预前后感染细胞HCMV晚期mRNA的表达水平,观察感染细胞的病变进展,并在体外实验的基础上将热毒清应用于HCMV活动性感染孕妇的临床治疗,测定治疗前后血清肿瘤坏死因子α(TNF-α)和白细胞介素2(IL-2)的水平。

AIM：To study the therapeutic effect of traditional Chinese medicine Reduqing on human cytomegalovirus （HCMV）. METHODS： The expres- sion of the HCMV late-mRNA in infected cells was measured by quantitative reverse transcription-polymerase chain reaction （RT-PCR） and the development of cytopathic effect （CPE） caused by HCMV was observed before and after treatment with Reduqing. On the basis of its anti-HCMV activity study in vitro, Reduqing was applied to the child-bearing aged women and pregnant women who were HCMV-IgM positive which indicated the active HCMV infection. The serum levels of tumor necrosis factor-alpha （TNF-α） and in- terleukin-2 （IL-2） before and after treatment were measured by the ELISA method. RESULTS：The results showed that the Reduqing had a strong inhibitory effect on both HCMV-mRNA and CPE. The level of the TNF-α increased while IL-2 decreased significantly in HCMV-IgM positive serum when compared with HCMV-IgM negative cases. In HCMV-IgM positive patients, after treatment with Reduqing, the serum levels of TNF-α and IL-2 returned to normal, and 73 % of them turned to be HCMV-IgM negative, which was significantly higher than that （ 14 % ） in control group. CONCLUSION：It is suggested that serum TNF-α and IL-2 level are closely related to the activity of HCMV. Reduqing can enhance the immunity of the body and has an obvious inhibition on HCMV in vitro and in vivo, and can thus serve as an effective medicine for actire HCMV infection therapy, especially for the pregnant women.