苯丁酸钠单用与氟尿嘧啶或顺铂联用对喉癌Hep-2细胞株的影响

Effects of sodium phenylbutyrate alone or in combination with fluorouracil or cisplatin on laryngeal carcinoma Hep-2 cell line

目的:研究苯丁酸钠(sodiumphenylbu-tyrate,PB)对体外喉癌Hep-2细胞株生长和分化的影响,及其能否增强氟尿嘧啶、顺铂对喉癌细胞的细胞毒性。方法:采用流式细胞仪检测PB作用后喉癌细胞的细胞周期,应用四氮唑蓝法检测PB单独及联用氟尿嘧啶或顺铂时,喉癌细胞的存活能力。结果:经PB作用4d后,细胞周期出现G1-S期阻滞和剂量依赖性生长抑制作用;PB的半数抑制浓度(IC50)是4.21mmol·L-1。1mmol·L-1PB与氟尿嘧啶或顺铂联用3d后,2药的IC50分别由(11.4±s1.7)mg·L-1和(1.60±0.05)mg·L-1下降至(8.2±0.8)mg·L-1和(1.36±0.11)mg·L-1P<0.05。结论:PB能诱导体外喉癌细胞发生分化和生长抑制作用,增强氟尿嘧啶、顺铂对喉癌细胞株的细胞毒性作用。

AIM： To study effects of sodium phenylbutyrate （PB） on the proliferation and differentiation of laryngeal carcinoma Hep-2 cell line in vitro. Synergistic induction of apoptosis in combination with fluorouracil or cisplatin. METHODS： The cell cycle of laryngeal carcinoma after PB treatment was measured by using flow cytometry and the viability was measured by the 3-（ 4,5-dimethylthiazol-2-yl ） -2,5-diphenyltetrazolium bromide assay （MTF） after single PB or in combination with fluorouracil or cisplatin management. RESULTS：After treating with PB for 4 d, the Hep-2 cell line showed a G1-S-phase block and a dose-dependent growth arrest. The IC50 of PB was 4.21 mmol ·L^-1 The IC50 of fluorouracil or cisplatin descended to （8.2 ±s 0.8） mg · L^-1 and （1.36 ±0.11） mg · L^-1 from （11.4±1.7） mg · L^-1 and （1.60±0.05） mg ·L^-1 respectively when they were combined in use with PB respectively. CONCLUSION： PB could induce differentiation and growth inhibition of laryngeal carcinoma Hep-2 cell line in vitro and enhance the cytotoxic effects of fluorouracid, cisplatin used in the treatment of laryngeal carcinoma.