摘要
合成了一种未见报道的铜(Ⅱ)苏氨酸邻菲咯啉配合物。根据元素分析、红外光谱、差热热重分析对其进行表征;并以电子吸收光谱法及溴化乙锭(EB)荧光分析法研究了此种配合物与DNA的作用。结果表明:此种配合物与DNA作用时,其电子吸收光谱的最大吸收峰明显红移,并产生明显的减色效应,同时,配合物也能较大程度地猝灭EBDNA体系的荧光,表明此种配合物与DNA存在很明显的插入结合作用。
A new complex [ Cu （Thr） （Phen）H2O]SO4· H2O· CH3OH （Thr = DL-Threonine, Phen = o-Phenanthroline）, which has not been published, was synthesized and characterized by elemental analysis, IR spectroscopy, and TG-DTA. The interaction of the complex and sperm DNA was studied by electronic absorption and ethidiurn bromide （EB） fluorescence spectroscopy. The result indicates that the maximal absorption peaks of this complex are red-shifted and the intensity is weakened; At the same time, it can to some extent quench the emission intensity of EB-DNA system. Therefore, the authors come to a conclusion that the interaction of this complex and sperm DNA is intercalation.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2005年第9期1439-1442,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金(20371043)资助项目
