非小细胞肺癌中MMP-2、CD44V6的表达及其临床意义

Expression of CD44V6 and matrix metalloproteinase-2 innon-small cell lung cancer

目的探讨细胞黏附因子(CD 44V 6)和基质金属蛋白酶-2(MM P-2)在非小细胞肺癌(N SCLC)组织中的表达及其与肺癌浸润、转移和预后的关系。方法回顾性分析43例术前未进行放、化疗的肺癌患者的切除标本,采用免疫组化SP法检测肺癌组织中的CD 44V 6和MM P-2的表达。结果肺鳞癌与肺腺癌中CD 44V 6的阳性表达率分别为58.33%(14/24)和68.42%(13/19);MM P-2的阳性表达率分别为54.16%(13/24)和84.21%(16/19)。CD 44V 6与MM P-2的阳性表达与淋巴结转移、肺癌病理分期以及术后血行转移显著相关(P<0.05),CD 44V 6阳性表达者的3年生存率为25.62%,5年生存率为6.41%;阴性表达者的3年生存率为64.71%,5年生存率为56.82%,两者差异显著(P=0.000)。MM P-2阳性者的3年生存率为18.8%,5年生存率为8.67%;阴性表达者的3年生存率为66.30%,5年生存率为42.66%,两者差异显著(P=0.0067)。CD 44V 6和MM P-2的阳性表达呈显著性相关(P=0.007)。结论CD 44V 6和MM P-2对于肺癌的侵袭、淋巴结转移、术后血行转移以及预后有一定作用。

Objective.. To detect the expression of cell adhesion molecules-v6 （CD44V6） and matrix mettalloproteinase-2 （MMP-2） in non-small cell lung cancer （NSCLC）, and to explore their association with invasion metastasis and prognosis. Methods ： A rapid immunohistochemical method （streptoavidin-peroxidase SP） was used to detect CD44V6 and MMP-2 proteins expression in 43 cases of NSCLC ,these cases were not treated radiotherapy or chemotherapy before operation. Results ：The expression rates of CD44V6 in lung squamous cell carcinoma and lung adenocarcinoma were 58.33 %, 68.24 % respectively. The positive expression rates of MMP-2 in lung squamous cell carcinoma and lung adenocarcinoma were 54.16%, 84.21% respectively. The positive expression of MMP-2 and CD44V6 were significantly correlated with lympha node metastasis, TNM stage and postoperative hematogenous metastasis （ P 〈0. 05） The 3-year,5-year survival rates of CD44V6 positive groups were 25.62%, 6.41 % ,negative expression groups were 64.71%, 56.82 % ,The significant difference of survival time was displayed between CD44V6 positive and negative expression groups （P = 0. 000）. The 3-year, 5-year survival rates of MMP-2 positive expression were 18.8%, 8.60%, negative expression groups were 66.30% and 42.82% separatately,The significant difference was showed between MMP-2 positive expression and negative expression groups （ P = 0. 0067）. The positive expression of CD44V6 was significantly correlated with MMP-2 positive expression （ P = 0. 007）. Conclusion ：CD44V6 and MMP-2 may play a role in NSCLC invasion,lympha node metastasis,postoperative hematogenous metastasis and prognosis.