DCC法测定乳腺癌雌激素受体方法的研讨

AN INVESTIGATION OF DEXTRAN-COATED CHARCOAL ASSAY FOR ESTROGEN RECEPTOR IN BREAST CANCER

用DCC多点法和单点饱和法对32例乳腺癌标本做ER受体测定,并对两法做了仔细比较,发现它们的结果有相关性。用统计学处理比较两者之间的差别,发现:用秩和检验法得P>0.1,用配对t检验法得P>0.5,说明二者之间无显著差别。作者认为单点饱和法的优点是方法简便,需用瘤体组织少,成本低廉,因此认为DCC单点法是可取的。

A study on single-point dextran-coated charcoal method and multipoint Scatchard analysis for estrogen receptor assay in breast cancer was performed in 32 cases.Their results were carefully compared. It has been shown that the Single-pointassay for estrogen receptor has good correlation with values obtained from mul-tipoint Scatchard analysis in this present study. Stastically. there were no signi-ficant difference between the results of two methods (P>0.1 in rank sum test andP>0.5 in paired t-test). Advantages of the single-point assay are to simplifythe procedure, to reduce amount of tissue required and cost per assay. The authorstherefore, believe it is unnecessary to carry-out Scatchard plot analysis for everycase.