工蜂蛹及其提取物对慢性炎症和免疫性炎症的作用及机理

Effects and the action mechanism of worker bee pupa and its extract on chronic inflammation and immuno-inflammation

通过工蜂蛹及其水提物和醚提物对小鼠肉芽肿模型、大鼠佐剂性关节炎模型的作用和对肾上腺中VC及胆固醇含量的影响实验,研究工蜂蛹对慢性炎症和免疫性炎症的作用及其机理.结果发现,工蜂蛹及其中、高剂量的水提物均对小鼠棉球肉芽肿有显著的抑制作用,对大鼠佐剂型关节炎模型的原发性病变和继发性病变具良好的对抗作用,能抑制细胞因子IL-6及炎症介质PGE2的生成,明显改变肾上腺中的胆固醇和VC含量,并能有效地防止造模期间体重的减轻,且对肾上腺和胸腺指数无明显影响;但中高剂量的醚提物对模型的影响效果不明显.表明工蜂蛹发挥抗炎及免疫作用的大部分活性物质可能存在于其水提物中,其作用机制可能是通过作用于肾上腺皮质系统而发挥抗炎作用.抑制单核巨嗜细胞的活化和分化,抑制炎症介质的产生是工蜂蛹发挥抗炎和免疫调节作用的机理之一.

To elacidate the action mechanism of worker bee pupae （WBP） on chronic inflammation and immuoiflammation, effect of WBP and its water extract and ether extract on granuloma mouse, complete Freund＇s adjuvant induced arthritis rats and Vit C and cholesterol content in normal adrenal glands of rats were examined. The results showed that WBP and its water extract at medium and high dosage produced a prominent inhibitory effect on granuloma in mouse, brought about antagonism against primary and secondary lesion in arthritis rats significantly, restrained increase in amount of IL-6 and PGE2, prevented decrease in body weight of rats in the course of establishing model, changed the level of Vitamin C and cholesterol in normal adrenal glands of rats distinctly, and index of thymus, adrenal glands were not affected apparently; effect of ether extraction of WBP was less remarkable than that of WBP and its water extract. Therefore, the active ingredients of WBP were believed to be water soluble and enter into water extract. The anti-inflammatory effect seemed to be produced through their influence on adrenal cortex system. Inhibition of activation and differentiation of mononuclear phagocyte as well as increase inflammatory medium may be one of the possible action mechanisms of immune-regulation and anti-inflammatory action of WBP.