黄藤遗传多样性的RAPD分析

Study on Genetic Diversity of Daemonorops margaritae Using RAPD

利用RAPD标记从DNA水平对收集的4个天然黄藤种群进行遗传多样性分析。15条引物扩增得到154条片段,整个种的多态性位点比率为75.97%,Ne i’s指数为0.258 4,Shannon信息指数为0.388 8。黄藤种的遗传多样性为0.257 8,种群间的Ne i遗传分化系数为0.141 2,各种群间的遗传变异非常小。4个种群间的遗传相似性分析结果显示:毛感乡和尖峰岭种群间的遗传相似度最高,吊罗山和坝王岭种群间的遗传相似度最低。AMOVA分析结果表明:种群间基因分化系数为0.026 2,大部分遗传变异(97.38%)来源于群体内。研究结果揭示:收集的天然黄藤种群具有较高的遗传多样性,有良好的保存和利用价值,尖峰岭种群可以作为重点保护。

The paper analyzed the genetic diversity of Daemonorops margaritae （Hance） Beccari using RAPD, based on four natural populations. A total of 154 sites were detected by 15 primers, which the proportion of polymorphic loci was 75.97%. The Nei＇ s index and Shannon information index were 0. 258 4 ＇and 0. 388 8, and total genetic diversity was 0. 257 8. Genetic diversity of D. margaritae mainly distributed within population, which the coefficient of gene differentiation was 0. 141 2. Genetic identity between Maoganxiang and Jianfengling population was the highest while that between Diaoluoshan and Bawangling population was the lowest. The results of AMOVA indicated that most of the variation （97.38%） resided within population. It was concluded from present study that there existed high genetic diversity of D. margaritae genetic resources, especially in Jianfengling population, which should be the priority population to be protected.