银杏苯丙氨酸解氨酶基因的克隆和序列分析

Molecular Cloning of Phenylalanine Ammonia-lyase in Ginkgo biloba

根据其他植物PAL基因的DNA序列的保守区域,设计了一对简并引物,经PCR扩增,得到一条862 bp的特异性扩增带。将PCR扩增产物构建到T-载体并测序,获得了862 bp DNA序列。通过分析所得的862 bp DNA序列及其编码的氨基酸序列,与其他植物PAL基因的DNA序列和蛋白质序列分别进行比较,证实了所得序列为银杏PAL基因的部分序列。Gbpal1已经被Genebank收录,序列号为AY578145。Southern杂交结果表明银杏PAL是一个多基因家族。

According to the conservative domain of some other plants DNA sequences of PAL gene, a pair of degenerate primer was designed. A special DNA fragment was obtained by PCR amplifying, which length is 862 bp, and it is named Gbpal1. We analysed the nucleotide and amino acid sequence of smilarity to the corresponding gene of other organism, the resuits showed that Gbpall is a partial PAL gene of Ginkgo biloba. The sequence had been embodied in Genebank, and Accession NO. is AY578145. The southern blot analysis indicated that Ginkgo biloba PAL belonged to a multigene family.