点燃癫痫模型基因的差异表达及草果知母汤对其影响

Differential Expression and Influence of Caoguo Zhimu Decoction on Kindling Epilepsy Related Genes

目的筛选与点燃癫痫模型发病及草果知母汤发挥疗效相关联的基因。方法使用mRNA基因差异显示技术(DDRTPCR),筛选差异基因条带,并使用Northern印记杂交排除假阳性。结果正常对照组、模型组、中药组存在110条差异条带,其中11条基因表达有明显差异的片段在正常对照组不存在,但出现在模型组,又在治疗组消失,说明这11条基因是与癫痫发病和治疗密切关联的基因。经NCBI美国国家基因库检索,这11条基因中的7条为同源基因,有4条基因片段为功能未知的新基因,其中3条新基因经Northern印记杂交排除了假阳性的可能,已经在GeneBank进行了注册,注册号分别CK325391、CK325392、CK325393、CK325394。结论草果知母汤可能通过影响海马组织部分基因片段的表达而起到治疗癫痫模型的作用。

Objective To screen the relative genes associated with the genesis of kindling epilepsy and the effect of Caoguo Zhimu Decoction （CZD） on these genes. Methods mRNA differential display reverse transcription PCR was used to screen differential gene bind, and Northern blotting hybridization was applied to exclude the false positive reaction. Results There were as more as 110 differential expression bindings in the normal group, model group and the treated group, among them 11 binds with differential expression only appeared in the model group but not in the normal group and disappeared in the treated group. It indicated that the 11 genes are correlated with the genesis and treatment of kindling epilepsy. By searching in the Gene-bank of NCBI, 7 bindings of the 11 were homologous genes and 4 gene fragments are novel genes with unknown function, which have been registered at the Gene-bank, with the registered numbers of CK325391, CK325392, CK325393, CK325394, respectively, and false positive possibility of 3 novel genes was excluded. Conclusion CZD has effect in treating kindling epilepsy may be through influencing the expression of partial gene fragments in the hippocampus.