青蒿琥酯对K562细胞凋亡诱导作用及其与亚砷酸的协同作用

Apoptosis-inducing effect of artesunate on K562 cells and its synergy with arsenious acid

目的研究青蒿琥酯体外诱导K562细胞凋亡的作用及其与亚砷酸的协同作用。方法采用MTT法、透射电镜技术、流式细胞术检测青蒿琥酯或(和)亚砷酸作用后K562细胞的凋亡发生情况。结果青蒿琥酯抑制K562细胞的增殖,IC50值为12.41μg/mL,其有时间、剂量-效应关系;12.50～50.00μg/mL的青蒿琥酯可诱导K562细胞凋亡,并具有一定的时间剂量-效应关系;12.50μg/mL青蒿琥酯与1.00μmol/L亚砷酸联合作用于K562细胞48h后,凋亡率为21.07%,与阴性对照(1.87%)、单独应用青蒿琥酯(7.45%)、亚砷酸(2.57%)相比,差异有统计学意义,χ2依次为1128.39、335.47和1058.40,P值均为0.000。结论青蒿琥酯对K562细胞具有凋亡诱导作用,并且与亚砷酸具有协同作用。

OBJECTIVE： To study the apoptosis of K562 cells induced by artesunate in vitro, and its syrergy with arsenious acid. METHODS： Cell viability was determined by MTT assay. The cell apoptosis reduced by artesunate and/or arsenious acid was detected by uhrastructural changes, and flow cytometry in K562 cells treated. RESULTS： The cell proliferation was inhibited by artesunate and IC50 at 48 h was 12.41 μg/mL. Artesunate induced K562 cells apoptosis at 12.50 - 50.00 μg/mL. Apoptotic ratios of K562 cells after treatment by artesunate 12. 50 μg/mL compound with arsenious acid 1.00μmol/L for 48 h was 21. 07%, which was significantly different from the control and any of the compound alone, X^2 = 1 128.39,335.47,1 058. 40, P=0. 000, 0. 000, 0. 000. CONCLUSIONS： Artesunate may induce the apoptosis of K562 cells in vitro. And the induction of apoptosis in K562 by artesunate compounding with arsenious acid has the synergistic action.