肌红蛋白-纳米氧化铝模板-金胶复合组装体的直接电化学与电催化

Direct Electrochemistry and Electrocatalysis of Myoglobin Immobilized on Nano-alumina-gold Colloid Assembly System

将肌红蛋白(Mb)固定在纳米氧化铝(AAO)模板-金胶复合组装体修饰玻碳电极表面,制得Mb/AAO/Au colloid/GC薄膜电极.在pH=5.4的HAc-NaAc缓冲溶液中,该薄膜电极于-0.21 V(vs.Ag/AgC l)处有一对准可逆的氧化还原峰,为Mb血红素辅基Fe(Ⅲ)/Fe(Ⅱ)电对的特征峰.在AAO/Au colloid薄膜的微环境中,Mb与玻碳电极间的电子传递明显加快,该Mb/AAO/Au colloid/GC薄膜电极还可用于过氧化氢和溶解氧的催化还原.

Mb/AAO/Au colloid/GC electrodes were made by immobilizing Mb on the surface of Au colloid modified glassy carbon electrode covered with AAO templates. The cyclic voltammgram of Mb/AAO/Au colloid/GC films shows a pair of well-defined and nearly reversible peaks at about -0.21 V（ vs. Ag/AgCl） in acetic acid-sodium acetate buffers with pH =5.4, being the characteristic peaks of the Mb heme Fe（ Ⅲ）/ Fe （ Ⅱ ） redox couples. In the microenvironment of the AAO/Au colloid film, the electron transfer between Mb and the electrode was greatly enhanced. The hydrogen peroxide and oxygen were catalytically reduced at Mb/AAO/Au colloid/GC electrodes.