视网膜增殖性疾病玻璃体中表皮生长因子的放射受体定量测定

QUANTITATIVEMEASUREMENTOFEGFINVITREOUSOFPROLIFERATIVERETINALDISEASESBYRADIORECEPTORASSAY

目的：了解增殖性糖尿病视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｄｉａｂｅｔｉｃｒｅｔｉｎｏｐａｔｈｙ，ＰＤＲ）、增殖性玻璃体视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅｖｉｔｒｅｏｒｅｔｉｎｏｐａｔｈｙ，ＰＶＲ）、视网膜中央静脉阻塞（ｃｅｎｔｒａｌｒｅｔｉｎａｌｖｅｉｎｏｃｃｌｕｓｉｏｎ，ＣＲＶＯ）等患者玻璃体和血清中表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ）的水平。方法：放射受体法定量测定患者和正常对照组共６７例玻璃体和血清中ＥＧＦ的含量。结果：患者组玻璃体中ＥＧＦ含量明显高于对照组（Ｐ＜０．００１），其中以ＰＶＲ组含量最高；玻璃体切割液ＥＧＦ水平低于玻璃体原液，反应６０％～６３％的原液水平，二者呈正相关。对照组和患者组血清中ＥＧＦ比较，ＰＤＲ组最高，其次为ＣＲＶＯ组，ＰＶＲ组低于对照组。结论：ＥＧＦ可能在这些疾病的病因病理中起一定作用；

PURPOSE:Tomeasuretheepidermalgrowthfactor(EGF)contentsinvitreousandserumsamplesinnormalsubjectsandpatientswithproliferativeretinaldiseases.METHODS:Usingradioreceptorassay(RRA)tomeasuretheEGFcontentsinvitreousandseruminpatientswithpro-liferativediabeticretinopathy(PDR)16cases,proliferativevitreoretinopathy(PVR)20cases,centralretinalveinocclusion(CRVO)16cases,otherretinalvasculardiseases5cases,andcontrols10cases.RESULTS:TheEGFlevelsinvitreousofthepatientgroupwereapparentlyhigherthanthoseofthecontrols(P<0.001).Amongpatientgroup,theEGFcontentsinvitrectomyfluidwaslowerthanthatoforiginalvitreous,reflectingabout60%～63%EGFlevelinoriginalvitreous,bothshowedpositivecorrelation.TocomparetheEGFcontentsinserumofpatientsandcontrols,theEGFcon-tentsinserumofPDRgroupincreasedsignificantly.InCRVOgrouptheEGFcontentsalsoin-creased,whileinPVRgroup,theEGFcontentswerelowerthanthoseofthecontrols.CONCLU-SIONS:TheincreasedEGFcontentsinvitreousofpatientgroupmayplayaroleinthepathogenesisofproliferativeretinaldiseases.TheRRAisasensitivemethodforquantitativemeasurementofgrowthfactor.