人食管上皮细胞原代培养方法的研究

Study of the better medium for the culture ofhuman esophageal epithelial cells

目的探讨适用于组织工程食管研究的食管上皮细胞培养方法。方法对比食管上皮细胞在无血清培养基K-SFM及含血清培养基DM EM中的生长情况。结果食管上皮细胞在无血清培养基K-SFM中生长快,细胞形态好,不易被成纤维细胞污染。结论应用无血清培养基K-SFM培养食管上皮细胞,方法简便,适合推广应用。

Objective： To search for a kind of better medium for the culture of human esophageal epithelial cells. Methods： Human esophageal epithelial cells were derived from surgically resected esophagus of patients with esophageal cancer. The growth and proliferation of esophageal epithelial cells in serum-free medium K-SFM to serum-contained medium DMEM were compared. Results： In the serum-free medium K-SFM, the esophageal epithelial cells grew faster, had better growth shape and were less liable to be contaminated by fibroblasts. Conclusion： For primary culture of human esophageal epithelial cells, the serum-free medium K-SFM is convenient and proper to be spread out.