基因芯片技术筛选卵巢癌紫杉醇耐药差异表达基因的研究

Study of the drug-resistance related genes of taxolin patients with ovarian carcinoma

目的应用基因芯片技术研究人卵巢癌紫杉醇耐药细胞株OC 3/T ax300与其敏感细胞系OC 3之间的基因表达谱差异,筛选耐药相关基因。方法分别提取OC 3/T ax300与OC 3细胞的总RNA并纯化mRNA;将等量的mRNA逆转录,以Cy5和Cy3标记的cDNA做探针,在B iostarH 140S基因表达谱芯片上进行杂交。扫描芯片荧光信号图像,用基因图像分析软件对扫描图像进行数字化处理和分析。结果共筛选出显著表达差异基因234种,其中217种基因表达水平下调,17种基因表达上调;下调基因主要为EB病毒编码核蛋白(EBNA-3)、信号蛋白(COP 9),上调的基因主要是酪氨酸激酶(JAK 2)、热休克蛋白(HSP s)、还原型辅酶烟胺腺嘌呤二核苷酸(NADH)等。结论本研究筛选出的基因可能为进一步探讨卵巢癌肿瘤细胞耐药机制提供新的途径。

Objective, To study the gene differential expression of taxol resistance cell line OC3/Tax300 of ovarian carcinoma compared with other parent cell OC3 and to screen drug-resistance related gene by eDNA microarray. Methods： The cDNA retro-transcribed from equal quantity mRNA derived from OC3/Tax300 and OC3 which were labeled with Cy5 and Cy3 fluorescence as probe respectively. The mixed probe were hybridized with BiostarH140S gene expression chip. The acquired image was analyzed by software. Results： 234 signitificantly differently expressed genes were screened out, of which up- and down-regulated genes were 17 and 217 respectively. They were related to 14 kinds of genes. The down-regulated genes were EBNA-3, COP9, StIP1, and so on. The up-regulated genes were JAK2, HSPs, NADH, and so on. Conclusion： These genes may be related to the mechnisms of drug-resistance of ovarian cancer.