聚合酶链反应及血清学检测在肺炎支原体感染诊断中的意义

EVALUATION OF POLYMERASE CHAIN REACTION AND SEROLOGICAL TESTS IN THE DIAGNOSIS OF THE INFECTION BY MYLOPLASMA PNEUMONIA

本文对140例下呼吸道感染患儿进行痰MP-DNA-PCR、抗MP-IgM及冷凝集试验检查。其中MP-DNA-PCR阳性52例,抗MP-IgM阳性19例,冷凝集试验阳性7例。结合临床诊断为MP感染60例。结果显示痰MP-DNA-PCR检查明显优于特异性血清抗MP-IgM抗体检测(P<0.001),而抗MP-IgM抗体检测又较传统冷凝集试验敏感(P<0.05)。本资料提示应用痰MP-DNA-PCR检测技术可为MP肺炎提供早期、快速诊断的途径,PCR配合血清抗体检测可减少MP感染漏诊率。

This article analyses 140 children infected with their lower respiratory tracts with the test of suptum MP-DNA-PCR, anti-MP-IgM and cold agglutination combined with clinical examination. Sixty of the children are diagnosed as MP infection among which 52 of them are MP-DNA-PCR positive, 19 are anti-MP-IgM positive and 7 are cold agglutination test posi tire. The result shows that the test of MP-DNA-PCR is apparently advanced than specific anti-MP-IgM serum antibody test (P<0.001)and anti-MP-IgM test is more sensible than traditional cold agglutination test(P<0.05). The information indieates that it supplies early and rapid diagnosis for MP pneumonia with the technic of sputum MP-DNA-PCR test and it reduces the rate of false diagnosis for MP infection with PCR and serum antibody test.