盐酸胺碘酮在玻碳电极上的电化学行为研究

Study on the electrochemical behavior of amiodarone at glassy carbon electrode

采用循环伏安法、线性扫描伏安法、微分脉冲伏安法、计时库仑法和恒电位库仑法详细研究了盐酸胺碘酮的电化学行为.结果表明,在微分脉冲伏安模式下,盐酸胺碘酮在pH 4.65的B-R缓冲液中,在+0.85 V(vs.Ag/AgCl)电位处产生一灵敏的阳极氧化峰,该峰电流值与盐酸胺碘酮的浓度在2.0×10-7～4.0× 10-6 mol/L范围内呈良好的线性关系,据此建立了微分脉冲伏安法检测盐酸胺碘酮的方法,其最低检测限为3.3 × 10-8 mol/L,该法应用于兔子血清及尿样中盐酸胺碘酮的测定,回收率达到91.1%～94.0%.并详细讨论了盐酸胺碘酮在玻碳电极上的电化学反应机理.

The electrochemical behavior of amiodarone on a glassy carbon electrode was investigated by cyclic voltammetry, linear sweep voltammetry, chronocoulometry and bulk electrolysis with coulometry. It was found that amiodarone would give a sensitive oxidation peak at ＋0.85V in the BR buffer solution （pH 4.65） under the differential pulse voltammetric （DPV） mode. The peak current was linear with the concentration of amiodarone in the range of 2.0×10^-7-4.0×10^-6 mol/L. Based on which, a DPV method for determination of amiodarone with the detection limit of 3.3×10^-8 mol/L has been developed. The proposed method has been used for determination of amiodarone in the rabbit serum and urine sample, the recovery was found to be in the range of 91.1%-94.0 %. The mechanism for this electrochemical reaction at the glassy carbon electrode was also discussed in this paper.