结直肠癌中Neu3基因的表达及产物活性的检测

Expression of neu3 gene and assessment of sialidase activity in human colorectal cancer

目的探讨神经苷节脂特异性的唾液酸酶(Neu3)基因与人类结直肠癌的关系。方法用逆转录聚合酶链反应(RTPCR)方法检测8例结直肠癌组织和正常黏膜之间Neu3mRNA的表达差异;用硫巴比妥酸方法(TBA)检测32例结直肠癌组织和正常黏膜中唾液酸酶活性。结果在转录水平,8对结直肠癌组织和正常组织中,癌组织中Neu3mRNA的表达明显高于正常组织中Neu3mRNA的表达水平;在蛋白质翻译水平,32例结直肠癌组织中的唾液酸酶活性平均为10.60U/mg,32例正常黏膜组织中的唾液酸酶活性平均为5.51U/mg(P<0.05)。结论Neu3基因的编码产物神经苷节脂特异性的唾液酸酶与结直肠癌细胞的分化和生长密切相关。Neu3基因可能与人类结直肠癌有关。

Objective To study the relationship between human plasma membrane-associated sialidase （Neu3） and human colorectal cancer. Methods The Neu3 expression difference of mRNA for this sialidase was detected by using RT-PCR assays between human colorectal cancer and adjacent non-tu- mor mucosa （ n = 8）. The activities of the sialidase were measured with ganglioside as the substrate in hu- man colorectal cancer and their adjacent non-tumor mucosa （ n = 32） by using 2-thiobarbitoric acid method （TBA）. Results The Neu3 mRNA expression was significantly increased in all of the cases for matched tumor as compared with adjacent non-tumor mucosa. Sialidase activity in 32 paired tumor tissues and adjacent non-tumor mucosa was 10.60 U/mg and 5.51 U/mg respectively （P 〈 0.05）. Conclusion Neu3 is significantly up-regulated in colorectal cancer and this finding provides a possible sialidase target for diagnosis and therapy of colorectal cancer.