尿多酸肽对乳腺癌细胞周期及Cyclin D1表达的影响

Effect of uroacitides on the cell cycle progression and the expression of Cyclin D1 of breast cancer cells

目的探讨肿瘤细胞分化诱导剂尿多酸肽(CDAⅡ)对乳腺癌细胞周期分布和CyclinD1表达的的影响。方法将CDAⅡ与不同生物学特性的乳腺癌细胞株MCF7和MDAMB231进行体外培养,分成3组:对照组、CDAⅡ组和维甲酸(ATRA)组,观察CDAⅡ对乳腺癌细胞生长曲线、细胞周期、CyclinD1表达及形态学等方面的影响。结果CDAⅡ可减缓2株乳腺癌细胞的生长和增殖能力,改变细胞周期分布,使MCF7细胞株对照组、CDAⅡ组和ATRA组S期比例分别为33.04%、21.70%和23.64%;MDAMB231细胞分别为38.7%、28.7%和27.9%;MCF7细胞株对照组、CDAⅡ组和ATRA组细胞CyclinD1表达(荧光强度)分别为12.26、6.46和6.74,MDAMB231细胞分别为12.43、8.32和8.97。结论CDAⅡ可抑制不同生物学特性的2株乳腺癌细胞的生长和增殖能力,使细胞周期出现G0/G1期阻滞,并减少乳腺癌细胞CyclinD1表达。

Objective To investigate the effect of CDA-Ⅱ on the cell cycle progression and the expression of Cyclin D1 of breast cancer cells. Methods The breast carcinoma cell lines MCF-7 and MDA-MB-231 were divided into three groups （control, CDA-Ⅱ and ATRA）and cultured in vitro. The effects of CDA- Ⅱ on growth curve, cell cycle progression, the expression of Cyclin D1 and morphology were observed. MCF-7 and MDA-MB-231 have their different biologic characteristics. Results CDA-Ⅱ could suppress the growth, proliferation ability of MCF-7 and MDA-MB-231, and the rate of S phase in group control, CDA-Ⅱ and ATRA in MCF-7 was 33,04 %, 21,70 % and 23,64 % respectively, and that of MDA-MB-231 was 38.7%, 28.7% and 27,9% respectively,The Cyclin D1 expression in group control, CDA-Ⅱ and ATRA in MCF-7 was 12.26, 6,46 and 6.74 respectively, and that of MDA-MB-231 was 12.43, 8.32, 8,97 respectively, Conclusion CDA-Ⅱ has remarkable effect of anti-cell-proliferation and can induce cell cycle block of G0/G1 and decrease expression of Cyclin D1 on breast cancer cells.