肺癌患者血清中sCD44V6含量及内镜活检组织中CD44V6表达的临床意义

The clinical significance of content of serum CD44V6 and expression of CD44V6 protein in pulmonary carcinoma tissue from endoscope biopsy

目的探讨肺癌患者血清中可溶性CD44V6(sCD44V6)蛋白含量及活检癌组织中CD44V6蛋白表达的临床意义。方法应用酶联免疫吸附实验(ELISA)检测130例肺癌手术前后、32例健康人和116例随访病例血清中sCD44V6;应用生物素抗生物素免疫组织化学法(ABC法),检测所有肺癌患者经内镜活检癌组织内CD44V6蛋白表达状况。结果肺癌患者血清中CD44V6含量(3.58±0.56)明显高于健康对照组(1.59±0.44),术前(3.58±0.56)高于术后(1.78±0.52),复发转移组(3.16±0.54)高于无复发转移组(1.29±0.61);肺癌活检组织CD44V6蛋白表达率69.23%,与肺癌T分期无关,N0期CD44V6蛋白阳性表达率57.65%,N1期CD44V6蛋白阳性表达率92.93%,两者差异有统计学意义(P<0.01)。结论血清中CD44V6含量及经内镜活检的肺癌组织中CD44V6蛋白表达与肺癌的转移复发有关,可作为一种可靠的判断手术疗效、预后及复发转移的临床检测指标。

Objective To investigate the clinical significance of soluble serum CIM4V6 and CD44V6 protein in pulmonary carcinoma tissue from endoscope biopsy. Methods The serum CD44V6 content was detected by using enzyme-linked immunosorbentassay methods （ELISA） from 130 cases of pulmonary carcinoma （ pre- and post-operation）, 32 cases of healthy controls and 116 cases of follow-up visits, and the CD44V6 protein in pulmonary carcinoma tissue from endoscope biopsy by the avidin-biotin complex （ABC） immunohistochemistry technique. Results The serum content of CD44V6 was markedly higher in pulmonary carcinoma patients （3.58 ± 0.56） than that in healthy controls （ 1.59 ± 0.44）, higher befor operation （3.58 ± 0.56） than after operation （1.78 ± 0.52）, and higher in recurrence or metastasis groups （3.16±0.54） than in no recurrence or metastasis groups （1.29±0.61）. The CD44V6 protein expression in pulmonary carcinoma tissue was 69.23 %. There was no relationship between the expression of CD44V6 and the clinical stages of pulmonary carcinoma. The CD44V6 protein expression in NO and N1 stage pulmonary carcinoma was 57.65 % and 92.93 % respectively with the difference being significant between them （ P 〈 0.01 ）. Conclusion The serum content of CD44V6 and expression of CD44V6 protein in pulmonary carcinoma tissue from endoscope biopsy are related with the metastasis and recurrence of pulmonary carcinoma, and they may be used as a reliable marker to determine the postoperative effect, prognosis, recurrence and metastasis in clinical practice.