一种新的大鼠同种主动脉瓣异位移植模型

A new model for heterotopic aortic valve transplantation in the rat

目的探讨建立一种主动脉瓣异位移植动物模型,用于研究同种心脏瓣膜的保存。方法受体供体均采用雌性Wistar大鼠,各40只。整块切取受体心脏。保留完整的主动脉瓣、少量心室肌和部分二尖瓣前叶,构建移植物。将移植物与受体腹主动脉端侧吻合。结果术后28d,80%受体存活,无下肢瘫痪,移植物搏动良好,切开移植物肉眼观未见血栓,主动脉瓣位于正常位置且活动良好。受体死亡原因有血栓栓塞(12.5%)、术中出血(5.0%)和麻醉意外(2.5%)。结论此模型可广泛应用于同种心脏瓣膜保存方法等研究,是较理想的模型。

Objective To establish a more valuable animal model for heterotopic aortic valve transplantation in order to study the preservation of allograft valves. Methods In Wistar rats, the heart of the donor was removed en bloc. A ventricular myocardial cuff, an intact aortic valve and partial mitral valve were maintained and the composite allgraft was made up. The composite aUgraft was grafted heterotopically into the abdominal aorta of the recipient. Results On postoperative day 28, 80% of the recipients survived without the signs of ischemic disorders of the lower limbs and thrombosis in the aorta graft while pulsation of the aorta graft existed. Thromboembolism （12.5%）, bleeding during operation （5.0 % ） and anaesthetic accident （2.5 % ） were the causes of recipient death. Conclusion This ideal model for heterotopic aortic valve transplantation enables us to evaluate modalities of preservation for valves used as allografts.