氧化苦参碱的抗炎作用及其机制

Effect of oxymatrine on inflammation and its mechanism

目的:观察氧化苦参碱(OMT)的抗炎作用,并初步探讨其抗炎作用机制。方法:将50只雄性小鼠随机分成5组,即生理盐水组、阳性药地塞米松组及OMT 70、140和210 mg.kg-1组,应用二甲苯制备小鼠耳肿胀模型,以鼠耳肿胀程度作为观察指标;将48只Wistar大鼠随机分成6组,即生理盐水组、阳性药地塞米松组及OMT 50、100和150 mg.kg-1组,以及L-硝基-精氨酸甲酯(L-NAME)+OMT 100 mg.kg-1组,应用右旋糖苷制备大鼠足跖肿胀炎症模型,以大鼠足跖容积作为观察指标。结果:腹腔注射OMT 140及210 mg.kg-1能剂量依赖性地减轻二甲苯所致小鼠耳肿胀程度(P<0.05或P<0.01),OMT 100和150 mg.kg-1可显著减轻右旋糖苷所致大鼠足跖肿胀的程度,与生理盐水组比较,差异具有显著性(P<0.05)。给予L-NAME能增强OMT的抗炎作用。结论:OMT能剂量依赖性地对抗二甲苯所致小鼠耳肿胀及右旋糖苷所致的大鼠足跖肿胀,其抗炎作用可能不是完全通过NO-cGMP途径实现的。

Objective To investigate the preliminary effect of oxymatrine （OMT） on inflammation and its mechanism. Methods Fifty mice were randomly divided into 5 groups： normal saline group, dexamethasone group and OMT groups with different doses （70, 140, and 210 mg · kg^-1 ）. The extent of ear swelling was observed in inflammation model caused by dimethyl benzene in mice. Forty-eight Wistar rats were randomly divided into 6 groups ： normal saline group, dexamethasone group and OMT groups with different doses （50, 100, and 150 mg · kg ^-1）,L-NAME＋OMT 100 mg · kg^-1 group. Paw edema volume induced by dextran were observed in rats. Results The extents of ear swelling were decreased in dose-dependent manner after intraperitoneal injection of OMT 140 and 210 mg · kg^-1 in mice compared with control （P〈0.05 or P〈0. 01）. Paw edema volumes were reduced after intraperitoneal injection of OMT 100 and 150 mg · kg^-1 in rats compared with control （P〈0. 05）. L-NAME enhanced the effect of anti-inflammation of OMT. Conclusion OMT can reduce the extent of ear swelling induced by dimethylbenzene in mice and the paw edema volume caused by dextran in rats. Its anti-inflammatory effect may be not fully mediated by NC-cGMP pathway.