维A酸对人类恶性黑色素瘤A375细胞增殖的影响

Effects of retinoids on proliferation of human melanoma cell line A375

目的:研究维A酸(RA)抑制人类恶性黑色素瘤A375细胞增殖的作用.方法:用RA(atRA,13cisRA和9cisRA)、MA(RXR激动剂)及TTNPB(RAR激动剂)处理培养的人类恶性黑色素瘤A375细胞.用MTT法研究这些试剂对细胞增殖的影响,用流式细胞仪研究这些试剂对细胞周期的影响.结果:RA和TTNPB均可抑制A375细胞的增殖,而MA无此作用.在作用24h时的不同浓度(10-5,10-6和10-7mol/L)以及作用48和72h的10-5mol/L浓度时,TTNPB的抑制增殖作用均强于其它试剂处理组(P<0.05).细胞周期分析结果显示RA和TTNPB均可诱导A375细胞G0/G1期阻滞,而MA无此作用.在作用24和48h时的10-5mol/L浓度时,TTNPB的诱导G0/G1期阻滞作用均强于其它试剂处理组(P<0.05).结论:RA可抑制人类恶性黑色素瘤A375细胞的增殖,并诱导G0/G1期阻滞.RA的这些作用与RXR的激活无关,而可能与RAR的激活有关.

AIM： To investigate the effects of diverse retinoids and receptor agonists in inhibiting proliferation of human melanoma cell line A375. METHODS： Human melanoma cell line A375 was treated by diverse retinoids （at-RA, 13-cis RA and 9-cis RA）, MA （ RXR agonist） and TTNPB （ RAR agonist） and their effects on the proliferation and cell cycle of A375 cells were analyzed by MTT and flow cytometer. RESULTS： Both retinoids and TTNPB inhibited the proliferation of A375 cells, but MA had no such effect. At all concentrations （10^-5, 10^-6 and10^-7 mol/L） in 24 h and 10^-5 mol/L in 48 and 72 h, TTNPB had the most powerful effect in all reagents （ P 〈 0.05）. The results of cell cycle analysis showed that retinoids and TTNPB caused the blockage of G0/G1 phase, but MA had no such effect. At 10^-5 mol/L in 24 and 48 h, TTNPB had the most powerful effect of causing blockage of G0/G1 phase in all reagents （ P 〈0.05）. CONCLUSION： Retinoids can inhibit the proliferation and cause the blockage of G0/G1 phase in human melanoma cell line A375. It is not the activation of RXR but the activation of RAR that may be related to these effects.