海水亚硝酸氧化细菌初始富集过程中硝酸盐的定性检测

A QUALITATIVE DETECTING METHOD FOR NITRATE IN THE INITIAL ENRICHMENT SYSTEM OF MARINE NITRITE- OXIDIZING BACTERIA

本文对海水亚硝酸氧化细菌初始富集过程中硝酸盐的定性检测方法及其适用范围进行了研究。结果表明, 在NaNO2起始浓度为100mg/L的亚硝酸氧化细菌初始富集培养系统中,1mL培养液中残余的NaNO2,可先用 1．0mol/L盐酸溶液20μL和50g/L氨基磺酸铵溶液10-20μL将其去除,然后再用二苯胺试剂对培养液中经亚硝酸 氯化细菌转化来的NaNO3进行定性检测,可检测出的NaNO3浓度下限在20mg/L左右。在NaNO2起始浓度不同的 富集培养系统中,去除NaNO2所需盐酸溶液、氨基磺酸铵溶液的量可根据其起始浓度按比例相应增减,但NaNO2的 起始浓度不宜超过200mg/L。该方法亦适用于淡水亚硝酸氧化细菌初始富集培养过程中硝酸盐的定性检测。

In the enrichment system of marine nitrite-oxidizing bacteria with 100mg/L of initial concentration of NaNO2, in order to qualitatively detect the nitrate produced by nitrite-oxidizing bacteria, the residual nitrite must be removed firstly, which can be made successfully by adding 20μL of 1.0 mol/L HC1 and 10 ～ 20μL of 50g/L NH4SO3NH2 to per milliliter of media. Then, the nitrate can be qualitatively detected by using diphenylamine reagent. The minimal concentration of NaNO3 that can be detected is about 20mg/L. In the different enrichment system with different initial concentration of NaNO2, the dose of HC1 and NH4SO3NH2 used to remove NaNO2 can be regulated in proportion as the initial concentration of NaNO2 , but the maximum of its initial concentration should not surpass 200mg/L. Also, this kind of qualitative detecting method for nitrate can be applied in the initial enrichment system of freshwater nitrite-oxidizing bacteria.