传统二次离心法和改良法制备富血小板血浆的比较研究

A new simplified method of platelet-rich plasma preparation

目的筛选简便、有效、安全的方法获得富血小板血浆(platelet-richplasma,PRP),为局部骨缺损修复提供自体生长因子。方法采集8名志愿者肘静脉血,分别用传统法和改良法制备PRP,进行血细胞计数分析,并用ELISA法定量分析PRP来源的血清中TGF-β1浓度。结果改良法通过封闭容器制备得到PRP,其血小板浓度(P=0.003)、血小板富集系数(P=0.004)和血小板获得率(P=0.005)、TGF-β1浓度(P=0.041)、TGF-β1富集系数(P=0.028)均高于传统法。结论改良法可获得高质量的PRP并减少与外界接触污染的危险性。

Objective：To improve the method for isolating platelet-rieh plasma （PRP）. Methods：Whole blood was collected from 8 healthy donors and then PRP was separated by both the tube method and the syringe method.respectively. Samples were activated to get serum rich-in growth factors （SRGF）. Platelets in the SRGF were counted and the level of TGF-β1 was assayed by enzyme-linked immunosorbent assay （ELISA）. Results：The end product of syringe method has both a higher platelet count in PRP （P = 0. 003 ） and a higher level of TGF-β1 in SRGF（ P = 0. 041 ） than that of tube method. Conclusion：The syringe method is an effective method in preparation of PRP.