雌二醇对人涎腺腺样囊性癌细胞增殖和转移作用的研究

Effects of estradiol on the proliferation and metastasis of adenoid cystic carcinoma SAcc83 cells

目的根据雌二醇(E2)临床用药在体内的药物浓度,探讨E2对人涎腺腺样囊性癌细胞(SAcc83)增殖和转移的影响。方法通过MTT法、细胞分裂指数、流式细胞仪等方法研究E2对SAcc83细胞增殖的影响;通过鸡胚心侵袭实验研究E2对SAcc83细胞的转移影响情况;通过对去势的雌裸鼠尾静脉注射方法建立动物转移模型和皮下致瘤实验,研究E2在动物体内对SAcc83的增殖和转移能力的影响。结果1×10-7、1×10-8、1×10-9mol/L的E2对SAcc83均有促增殖作用,其中10-7mol/L的E2作用最显著,作用1、2、3d时细胞分裂指数分别增加了33.3%,40.9%,17.8%,作用12、18、24h时S期细胞分别增加了12.3%,3.3%和9.7%。裸鼠皮下致瘤性实验中每只鼠每天皮下注射0.2ml9.55×10-5mol/LE2,注射10d,试验组肿瘤比对照组增加了24.8%。裸鼠肺转移实验中试验组裸鼠用药42d后,肺转移结节形成率增加了41.2%。结论10-7mol/L的E2对SAcc83细胞有明显的促增殖和转移作用,提示这类雌激素应在严密监控下使用。

Objective：To investigate the effects of 17-β estradiol （E2） on the proliferation and metastasis of adenoid cystic carcinoma SAcc83 cells. Methods：The effects of E2 on the proliferation of SAce83 cells were studied by MTY assay and flow eytometry. The invasion potential of the cells was investigated with chicken embryo heart invasion assay. The in vivo growth and metastasis of SAcc83 cell induced tumor were studied in ovary-reseeted female nude mice. Results：E2 at 10^-7,10^-8 and 10^-9 mol/L, promoted the proliferation of SAcc83 cells. E2 at 10^-7 mol/L showed the strongest growth stimulation effects and increased the cell proliferation by 34.4%. With exposure time of 1,2 and 3 d it increased the proliferation index by 33.3% ,40.9% and 17.8% respectively;with the exposure time of 12,18 and 24 h,it increased S phase cells in cell cycle by 12.3% ,3.3% and 9.7% respectively. Neoplasm developed in all nude mice after subcutaneous transplantation of 10^6 cells. In the mice treated with 0.2 ml of E2 at 9.55 × 10^ -5 mol/L every day for 15 days neoplasm volume doubling time was 61.5 h ,that in control mice 76.5 h. In lung metastasis trial, 42 days after tail injection of the cells the metastasis loci in E2 treated mice inreased by 41.2%. Conclusion： E2 may increase the proliferation and metastasis of adenoid cystic carcinoma cells.