TFAR19在口腔白斑和口腔鳞癌中表达的研究

The expression of TFAR19 in oral leukoplakia and oral squamous cell carcinoma

目的研究凋亡相关基因tfar19在口腔正常黏膜、口腔白斑和口腔鳞癌组织中的表达及意义。方法采用免疫组织化学方法检测17例正常口腔黏膜、60例口腔白斑,30例口腔鳞癌组织标本中TFAR19蛋白的表达。结果①口腔正常黏膜组TFAR19染色阳性率为88.2%,白斑组TFAR19染色阳性率为63.3%,口腔鳞癌组TFAR19染色阳性率为30%,3组间染色阳性率分别有统计学差异(分别为P≤0.05)。应用Crosstabs相关性统计分析,TFAR19染色阳性率与组织病变恶性度显负相关(r=-0.997,P<0.05)。②口腔正常黏膜、口腔白斑和口腔鳞癌组织的TFAR19细胞染色强度指数分别为199.34±25.33、130.23±19.21,和59.44±13.83,各组间分别有显著性差异。结论tfar19基因在口腔黏膜上皮成癌过程中的确发挥着重要的作用,并且可以推测tfar19基因的表达缺失发生在肿瘤形成的早期。

Objective：To study the expression and significance of apoptosls-related gene tfarl9 in normal oral mucosa, oral leukoplakia and oral squamous cell carcinoma. Methods：The expression of TFAR19 was observed in 17 cases of oral normal mucosa, 60 of oral leukoplakia and 30 of oral squamous cell carcinoma by immunohistochemical stain. Results：①Positive expression of TFAR19 was observed in 88.2% of the cases of oral normal mucosa, 63.3% of oral leukoplakia, and 30% of oral squamous cell carcinoma. There was statistical difference between each two groups （P 〈 0.05 ）. There was negative correlation between malignant degree of the lesions and positive rate of TFAR19 （ r = - 0. 997,P 〈 0.05 ）. ② Stainning intensity index （SⅡ） of TFAR19 was 199.34 ± 25.33 in the individuals with normal oral mucosa, 130.23 ± 19.21 in those with oral leukoplakia and 59. 44 ± 13.83 in those with oral squamous cell carcinoma. There was statistical difference between each two groups （P 〈 0.01 ）. Conclusion ：TFAR19 expression is negatively related to the development of oral squamous cell carcinoma.