Notch1、P21^(WAF1)和Involucrin在鼻咽癌组织中的表达及意义

Expression and Significance of Notch1, P21^(WAF1) and Involucrin in Nasopharyngeal Carcinoma

背景与目的:Notch1受体蛋白的异常表达常见于多种原发性肿瘤,但它与鼻咽癌的关系仍不清楚。本研究拟探讨鼻咽癌组织中Notch1受体蛋白、其下游蛋白P21WAF1和Involucrin蛋白的表达,以及它们与鼻咽癌分化程度的关系。方法:采用免疫组织化学SP法检测101例鼻咽癌组织和20例鼻咽慢性炎症组织中Notch1﹑P21WAF1和Involucrin蛋白的表达。结果:20例鼻咽慢性炎症组织中Notch1、P21WAF1和Involucrin蛋白表达率分别为100%(20/20)、90.0%(18/20)、100%(20/20);鼻咽癌组织中Notch1、P21WAF1和Involucrin蛋白的表达率分别为77.2%(78/101)、89.1%(90/101)和80.1%(81/101);三者在不同组织类型鼻咽癌组织中的表达均有显著差异,随着分化程度的下降表达明显减弱,分别为P=0.000,P=0.026和P=0.000;并且在角化性鳞状细胞癌、分化型非角化癌和未分化癌之间两两比较,表达均有显著差异,分别P<0.05;Notch1的表达水平分别与P21WAF1和Involucrin的表达水平正相关,P=0.002和P=0.001;而P21WAF1与Involucrin的表达水平无明显的相关。结论:鼻咽癌组织中Notch1、P21WAF1和Involucrin的表达与鼻咽癌细胞分化程度密切相关。

BACKGROUND ＆ OBJECTIVE： Abnormal expression of Notch1 protein was often found in many kinds of primary tumors, but its correlation with nasopharyngeal carcinoma （NPC） is still unclear. This study was designed to investigate the expression of Notch1 and its downstream proteins P21^WAF1 and Involucrin in NPC, and analyze their correlations with the differentiation of NPC cells. METHODS： The expression of Notch1, P21^WAF1, and Involucrin in 101 specimens of NPC and 20 specimens of chronic inflammatory nasopharyngeal mucosa were detected by SP immunohistochemistry. RESULTS： The positive rates of Notch1, P21^WAF1, and Involucrin were 100%, 90.0% , and 100% in chronic inflammatory nasopharyngeal mucosa, and were 77.2%, 89.1%, and 80.1% in NPC, respectively. The expression of Notch1, P21^WAF1, and Involucrin were significantly suppressed along with descending differentiation of NPC （P= 0.000, P=0.026, and P=0.000）. The positive rates of Notch1, P21^WAF1, and Involucrin were significantly higher in keratinizing squamous cell carcinoma （KSCC） than in differentiated nonkeratinizing carcinoma （DNKC） and undifferentJatied carcinoma （UDC） （P〈0.05）, and were significantly higher in DNKC than in UDC （P〈0.05）. The expression of Notch1 in NPC was positively correlated with the expression of P21^WAF1 （r=0.306, P=0.002） and Involucrin （r= 0.325, P=0.001）. No significant correlation was found between the expression of P^WAF1 and Involucin. CONCLUSION： The expression of Notch1, P21^WAF1, and Involucrin are closely correlated to the differentiation of NPC cells.