人胸椎黄韧带BMP-2和TGF-β的基因表达增龄性变化

Gene expression of collagen BMP-2 and TGF-β in human lumbar ligamentum flavum

[目的]研究不同年龄组胸黄韧带中BMP-2和TGF-β基因表达规律.[方法]利用BMP-2和TGF-β cD-NA探针,对不同年龄组的黄韧带冰冻切片进行原位杂交,用VIDAS图像分析仪分析.[结果]20岁以下组,有明显的TGF-β表达,无BMP-2表达;21～40岁组,黄韧带的附着点处有少量的BMP-2和TGF-β阳性表达信号.41～60岁组,在附着点处TGF-β表达较弱,但BMP-2表达相对增强.61岁以上组,TGF-β无表达,附着点处有呈簇状BMP-2阳性表达信号.VIDAS图像分析显示各组间差异有显著性.[结论]随着年龄的增长,TGF-β表达逐渐减弱,在附着点处出现BMP-2的表达.

[ Objective] To observe the gene expression of collagen BMP-2 （bone morphogenetic protein-2） anti TGF-β（transforming growth factor-β） in human thoracic ligamentum flavum. [ Method] BMP-2 and TGF-β probes were labeled and in situ hybridization results were analyzed by VIDAS system. [Result] （ 1 ） Under 20 years group： the positive gene expression of TGF-β was evident, but it was negative to BMP-2. （2） 21～40 years group： there were a few positive signal of both BMP-2 and TGF-β on the insertion part. （3） 41～60 years group： there was only few TGF-β positive signal on insertion part, but BMP-2 gene expression was relatively high. （4） Over 61 years group： there was not TGF-β expression, but BMP-2 expression could only be seen on insertion part and junction part, and was low, but the cells were areatus. [ Conclusion] In normal ligamentum, there is expression of TGF-β gene, but it decreases with age ; with age advancing BMP-2 emerges and enhances in the insertion part of ligamentum flavum.