射频能量在治疗关节软骨退变中对软骨细胞及基质的影响

Effects of radiofrequency energy on degenerative articular

[目的]实验研究射频消融能量大小与关节软骨细胞及基质损伤效应之间的关系。[方法]建立牛膝关节软骨退变模型,模拟膝关节镜环境,射频消融气化仪在15、40、70W的不同能量设置条件下,处理牛膝关节退变软骨1min,同时设置对照组,共分4组,每组6份标本,随后行软骨组织块培养,HE染色,FDA/PI双荧光染色,GAG释出率。[结果]随着射频消融能量的增加,空泡率明显增加,软骨细胞死亡率明显增加,GAG释出率明显减少。[结论]随着射频消融输出能量的增加,软骨细胞死亡率明显增加,软骨基质损伤明显加重,射频消融能量大小与关节软骨细胞及基质的损伤呈正相关关系。

[ Objective] To study the effect of radiofrequency energy on chondrocytes and matrix of traumatic degenerative articular cartilage. [ Method ] Traumatic degenerative arthritis models were established in 24 bovine knees, which were divided into 4 groups ： 15w group （A） , 40w group （B） 70w group （ C ） and control group untreated （D） After the degenerative cartilages of group A, B, C were treated respectively for 1 min with 15w, 40w, 70w radiofrequeney energy, the cartilage blocks were cultured and examined with HE stain and FDA （flureseein diaeetate） /PI （propidium iodide） double fluorescent stain, and GAG （glueosaminegluean） release rate was measured. [ Result ] Followed the enhance of radiofrequeney energy strength, the vaeuolization rate and mortality rate of cartilage cells were eorrespondently increased （ P 〈 0. 05 ） , but GAG release rate was decreased （P 〈 0. 05 ）, [ Conclusion ] The results shows that there are positive correlation between the strength of radiofrequeney energy and degree of matrix damage as well as mortality of cartilage cells.