川芎嗪对哮喘大鼠气道壁嗜酸细胞浸润和转化生长因子-β_1表达的影响

Effect of ligustrazine on the infiltration of eosinophil and expression of transforming growth factor-β_1 in asthmatic rats

目的:观察川芎嗪对哮喘大鼠模型气道壁嗜酸性粒细胞(EOS)浸润、转化生长因子-β1(TGF-β1)含量的影响。方法:32只SD大鼠随机分成正常对照组、哮喘模型组和大、小剂量川芎嗪干预组,以卵蛋白致敏,并长期吸入激发诱喘制备大鼠哮喘模型,干预组每次雾化吸入前腹腔注射川芎嗪(分别为80mg/kg和40mg/kg),模型组以生理盐水代替。肺组织石蜡切片行TGF-β1免疫组化染色,并经计算机图像分析测定其含量,同时HE染色计数气道壁嗜酸性粒细胞(EOS)数。结果:模型组气道壁EOS计数及TGF-β1含量均高于正常对照组(P均<0.001);两种剂量川芎嗪干预组EOS计数均低于模型组(P<0.01,P<0.05),但大小剂量组间无差别(P>0.05);干预组TGF-β1含量均显著低于模型组(P均<0.001),大、小剂量组间差异有显著性(P<0.01);TGF-β1的表达与EOS计数呈显著正相关(rs=0.7180,P<0.01)。结论:川芎嗪可以减少气道壁EOS浸润和TGF-β1的表达,其降低TGF-β1表达的作用可能部分通过抑制EOS浸润而实现。

Objective： To observe the effect of ligustrazine on eosinophil infiltration and expression of TGF-β1 in asthmatic rats airway wall. Methods：Thirty-two SD rats were randomly divided into 4 groups： the normal group, the model group, the low- and high-dose of ligustrazine group, 8 rats in each group. The asthmatic model rat was established by repeated inhalation of ovalbulium. The changes of TGF-β1 content in the airway wall were measured by the computerized image analysis system, and eosinophis within airway wall were detected. Results： The wall contents of TGF-β1 and eosinophils in airway wall were significantly higher （P 〈 0.001 ）in the model group than that in the normal group. In Ligustrazine treatment groups , the contents of TGF-β1 were lower （P 〈 0.001 ）than that in the model group; eosinophils were lower too （P 〈 0.01,0.05）. A close correlation between contents of TGF-β1 and eosinophils was demonstrated （rs = 0.7180, P 〈 0.01 ）.Conclusion： Ligustrazine could reduce eosinophil infiltration and inhibit the expression of TGF-β1 in airway wall.