大鼠局灶性脑缺血后交叉性小脑失联络现象及川芎嗪的干预作用

Crossed cerebella diaschsis after focal cerebral ischemia and the interventional effects of ligustrazine

目的:观察大鼠局灶性脑缺血后小脑失联络现象及中药川芎嗪的干预作用。方法:实验于2000-01在苏州大学附属二院神内科实验室进行。取15只SD大鼠随机分成正常对照组、模型组和川芎嗪组3组,每组5只。①给药:川芎嗪组大鼠在造模前半小时,给予川芎嗪50mg/kg(2.5mL/kg)腹腔注射,其他2组不给药。②造模:模型组和川芎嗪组大鼠线栓法建立大鼠右侧大脑中动脉缺血模型,正常对照组不造模。③小脑脑血流量的测定:采用氢清除法测定,正常对照组测1次,其他2组大鼠再灌注1,3,6及24h时测定4次。结果:15只大鼠全部进入结果分析。①正常对照组小脑脑血流量:右侧和左侧无显著差异[(1.35±0.07),(1.33±0.06)mL(/g·min),P>0.05]。②模型组小脑脑血流量:造模后左侧迅速下降(P<0.01),特别是再灌注后1h,左侧显著低于右侧([0.88±0.10),(1.29±0.03)mL(/g·min),P<0.01]。③川芎嗪小脑脑血流量:右侧显著高于同时段模型组右侧(P<0.05或0.01),其中再灌注后1h最显著([1.00±0.03),(0.88±0.10)mL(/g·min),P<0.05]。结论:大鼠一侧大脑中动脉缺血后,可引起对侧小脑脑血流量序列性变化,川芎嗪可改善远隔区域脑灌注,在改善脑卒中预后中起一定作用。

AIM： To observe the crossed cerebellum diaschisis after focal cerebral ischemia and the interventional effects of ligustrazine. METHODS： The experiment was carried out in the laboratory, of Department of Neurology, the Second Affiliated Hospital of Suzhou University in January, 2001. Fifteen SD rats were randomly divided into normal eontrol group, model group and ligustrazine group with 5 rats in each group. ① Administration： The rats in the ligustrazine group were treated with intraperitoneal injection of 50 mg/kg ligustrazine （2.5 mL/kg） at half an hour before model establishment, and those in the other two groups were not given any treatment. ② Model establishment： Rats in the model group and ligustrazinc group were made into models of ischemia of right middle cerebral artery, by the method of thread oeclusion, and those in the normal control group were not made .into models.③ Detection of cerebral blood flow of cerebellum： It was detected with the method of hydrogen elimination for only once in the normal control group and 4 times in the other 2 groups at 1, 3, 6 and 24 hours after reperfusion respectively. RESULTS： All the 15 rats were involved in the analysis of results. ① In the normal group, the cerebral blood flow had no difference between left and right cerebellum [（1.35±0.07）, （1.33±0.06） mL/g per minute, P 〉 0.05].② In the model group, the cerebral blood flow was rapidly decreased in the left cerebellum, especially after reperfusion for 1 hour, and it wag significantly lower in left eerebellum than in right cerebellum [（0.88±0.10）, （1.29±0.03） mL/g per minute, P 〈 0.04]. ③ In the ligustrazine group, the cerebral blood flow was significantly higher in right cerebellum than in left cerebellum at the same time period （P 〈 0.05 or 0.01）, it was the most significant at 1 hour after reperfusion [（1.00±0.03）, （0.88±0.10） mL/g per minute, P 〈 0.051. CONCLUSION： Unilateral middle cerebral artery, occlusion may influence the cerebral blood flow of erossed cerebellum. Ligustrazine can improve the cerebral reperfusion in the remote areas, and plays a certain role in the ameliorating the post-stroke clinieal prognosis.