摘要
为了进一步明确蛇龙珠品种的来源和亲缘关系,利用36个RAPD引物对14个酿酒葡萄品种基因组DNA扩增,通过聚类分析将其划分为4个类群,其中赤霞珠、品丽珠、美乐和蛇龙珠同属于一个类群.利用SSR技术对该类群(含3个不同地区来源的蛇龙珠)进一步分析结果表明:蛇龙珠、赤霞珠、品丽珠和美乐是4个独立的品种,遗传距离最大的是品丽珠和赤霞珠,为3.46,遗传距离最近的是美乐与品丽珠,遗传距离为2.40.就蛇龙珠不同类型而言,3个不同地区来源的蛇龙珠遗传上存在差异(欧氏遗传距离在1.41~1.73之间),但明显小于与其他品种的差异.与其他品种相比,3个地区的蛇龙珠都与品丽珠遗传距离最近(2.45~2.64),都与赤霞珠最远(2.83~3.32).
‘Cabernet Shelongzhu' is an important grape cultivar in China used for wine. However, its genetic origin is not clear yet. In this study, depending on the RAPD ( Random Amplification Polymorphism DNA) analysis with 36 primers, 14 wine grape cultivars were classified into four groups by UPGMA (unweighted pair group method using arithmetic average) cluster analysis. Four cultivars of them, i. e. Cabernet Sauvignon, Cabernet Shelongzhu, Cabernet Franc and Merlot, belonged to the same group. The SSR (Simple Sequence Repeat) analysis further indicated that they were four independent cultivars. Also, the results from SSR analysis showed that three phenotypes of ‘Cabernet Shelongzhu' sampled from different regions were genetically distinct with Euclidean distances ranging from 1.41 to 1.73, which were much smaller than those among distinct cultivars. Comparing with other cultivars, the phenotype of Cabernet Shelongzhu from Beigou at Penglai was the closest to Cabernet Franc with the Euclidean distance of 2. 45, and followed by Changli and Yantai with the Euclidean distances of 2. 56 and 2.64, respectively. The historical origin of the cultivar ‘Cabernet Shelongzhu' was investigated and discussed.
出处
《园艺学报》
CAS
CSCD
北大核心
2005年第4期604-608,共5页
Acta Horticulturae Sinica