西瓜杂交种遗传多态性的SRAP标记分析

Studies on Genetic Diversity with a Molecular Marker SRAP of Watermelon Hybrids

利用新型分子标记SRAP(Sequence-Related Amplified Polymorphism)进行西瓜杂交种遗传多态性的研究,利用25个引物组合对当前生产上推广的20个西瓜杂交种进行扩增,从中筛选得到20个多态性引物组合,共产生135个多态性条带,平均每个引物组合产生7.11个多态性条带,显示了较高的多态性比率.聚类分析20个引物组合的扩增结果,20份材料分为3大类,Jaccard's相似系数在0.29～0.86之间.

Study on genetic diversity of watermelon could benefit the research of cultivar property right protecting, seed purity testing, and genetic breeding. A new molecular marker system-SRAP （Sequence-Related Amplified Polymorphism） was firstly applied on 20 watermelon hybrids genetic diversity research. 2 primer pairs selected from 25 amplified 135 polymorphic bands with an average of 7.11 polymorphic bands pq primer pair. Cluster analysis using UPGMA method based on the data of SRAP amplified bands by 20 prirs, pairs showed 20 hybrids could be distinguished into three main groups. Jaccard＇s similarity coefficient range from 0. 29 -0. 86.